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재조합
expressed in E. coli
Quality Level
형태
liquid
특이 활성도
≥10,000 units/mL
≥5,000 units/mg protein
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
기술
molecular cloning: suitable
외래 활성
Nuclease-free
배송 상태
wet ice
저장 온도
−20°C
일반 설명
Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis on DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.
애플리케이션
HIV Reverse Transcriptase, Recombinant, E.coli has been used as a standard in reverse transcriptase activity assay.
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
단위 정의
One unit is defined as the amount of enzyme that will incorporate 1.0 nmol of labeled dTTP into acid-insoluble material in 20 min at 37°C, pH 8.3.
물리적 형태
In 10 mM potassium phosphate, 1 mM DTT, 20% glycerol, pH 7.4.
재구성
Following initial thaw, aliquot and freeze (-20°C).
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Geraldine Vilmen et al.
mBio, e0275221-e0275221 (2022-01-12)
Infection of rhesus macaques with simian-human immunodeficiency viruses (SHIVs) is the preferred model system for vaccine development because SHIVs encode human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Envs)-a key target of HIV-1 neutralizing antibodies. Since the goal of vaccines
Identification of an antiretroviral small molecule that appears to be a host-targeting inhibitor of HIV-1 assembly
Reed JC, et al.
Journal of Virology, 95(3), 10-1128 (2021)
Conformation of HIV-1 Envelope governs rhesus CD4 usage and simian-human immunodeficiency virus replication
Vilmen G, et al.
mBio, 13(1), e02752-e02721 (2022)
Bo Meng et al.
Cell reports, 35(13), 109292-109292 (2021-06-25)
We report severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike ΔH69/V70 in multiple independent lineages, often occurring after acquisition of receptor binding motif replacements such as N439K and Y453F, known to increase binding affinity to the ACE2 receptor and confer
Jonathan C Reed et al.
Journal of virology, 95(3) (2020-11-06)
Given the projected increase in multidrug-resistant HIV-1, there is an urgent need for development of antiretrovirals that act on virus life cycle stages not targeted by drugs currently in use. Host-targeting compounds are of particular interest because they can offer
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