추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, mouse
기술
immunoprecipitation (IP): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... LIN28A(79727)
일반 설명
Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.
특이성
Detects Lin28
Other species have not been tested.
면역원
Full-length His-tagged Lin28 recombinant protein (human).
애플리케이션
Anti-Lin28 Antibody detects level of Lin28 & has been published & validated for use in WB & IP.
Immunoprecipitation:
Independent lab validated this antibody in immunoprecipitation. The antibody immunoprecipitated Lin28 from Tera-2 embryonic carcinoma and mouse ES cells using 2μL of antibody per reaction.
Independent lab validated this antibody in immunoprecipitation. The antibody immunoprecipitated Lin28 from Tera-2 embryonic carcinoma and mouse ES cells using 2μL of antibody per reaction.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Signaling Neuroscience
RNA Metabolism & Binding Proteins
RNA Binding Protein (RBP)
Signaling Neuroscience
RNA Metabolism & Binding Proteins
RNA Binding Protein (RBP)
품질
Western Blot Analysis:
This lot detected Lin28 at 1:1,000 dilution in Tera-2 embryonic carcinoma cell lysate resolved via SDS-PAGE and transferred to PVDF.
This lot detected Lin28 at 1:1,000 dilution in Tera-2 embryonic carcinoma cell lysate resolved via SDS-PAGE and transferred to PVDF.
표적 설명
25 kDa
물리적 형태
Rabbit Serum
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt. Store at -80°C for longer term storage. Avoid repeated freeze/thaws.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Srinivas R Viswanathan et al.
Science (New York, N.Y.), 320(5872), 97-100 (2008-02-23)
MicroRNAs (miRNAs) play critical roles in development, and dysregulation of miRNA expression has been observed in human malignancies. Recent evidence suggests that the processing of several primary miRNA transcripts (pri-miRNAs) is blocked posttranscriptionally in embryonic stem cells, embryonal carcinoma cells
Yong Sun Lee et al.
The Journal of biological chemistry, 280(17), 16635-16641 (2005-02-22)
Micro-RNAs are small non-coding RNAs that regulate target gene expression post-transcriptionally through base pairing with the target messenger RNA. Functional characterization of micro-RNAs awaits robust experimental methods to knock-down a micro-RNA as well as to assay its function in vivo.
Geneviève Di Tomasso et al.
RNA (New York, N.Y.), 22(11), 1760-1770 (2016-09-24)
As part of their normal life cycle, most RNA molecules associate with several proteins that direct their fate and regulate their function. Here, we describe a novel method for identifying proteins that associate with a target RNA. The procedure is
Ligang Wu et al.
Molecular and cellular biology, 25(21), 9198-9208 (2005-10-18)
Vertebrate genomes each encode hundreds of micro-RNAs (miRNAs), yet for few of these miRNAs is there empirical evidence as to which mRNA(s) they regulate. Here we report the identification of human lin-28 mRNA as a regulatory target of human miR-125b
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