추천 제품
저장 온도
−20°C
일반 설명
The Hyas MicroMap Kit provides a method for small molecule intracellular target identification via photocatalytic proximity labeling. The kit contains a catalyst that can be coupled to an amine on almost any small-molecule ligand. The subsequent ligand-Iridium conjugate can be used to perform multiple target identification experiments. The following kit provides enough Ir-catalyst to synthesize 20 μMoles of iridium–ligand conjugate and enough Diazirine-PEG3-Biotin to perform 10 proteomics experiments.
The kit provides a step-by-step procedure for conjugating your ligand of choice to a photocatalyst that localizes light-mediated crosslinking to the ligands target. The ligand–iridium conjugate can then be incubated with live cells along with a biotinylated diazirine reagent. Analogous to a satellite, the photocatalyst localizes carbene generation from the diazirine only in the direct vicinity and thus covalently modifies the target protein in a catalytic manner, amplifying the signal for downstream analysis.
To link to an acid on a small molecule (instead of an amine), the Dione Kit is available.
Photoreactors commercially available:
The kit provides a step-by-step procedure for conjugating your ligand of choice to a photocatalyst that localizes light-mediated crosslinking to the ligands target. The ligand–iridium conjugate can then be incubated with live cells along with a biotinylated diazirine reagent. Analogous to a satellite, the photocatalyst localizes carbene generation from the diazirine only in the direct vicinity and thus covalently modifies the target protein in a catalytic manner, amplifying the signal for downstream analysis.
To link to an acid on a small molecule (instead of an amine), the Dione Kit is available.
Photoreactors commercially available:
- Penn PhD Photoreactor M2 (Z744035)
- Penn PhD Photoreactor M2 365nm Light Source (Z744031)
- LightOX PhotoReact 365 (Z744061)
Read more about: - Target identification with HyasKit and DioneKit in our Technology Spotlight
- Small molecule photocatalysis enables drug target identification via energy transfer
애플리케이션
Proximity protein labeling, protein-protein interactions, photoaffinity labeling, proteomics
생화학적/생리학적 작용
Kit of components packaged in individual vials for enabling photoinduced proximity labeling. The kit contains an Ir-catalyst to couple to an acid on a small molecule ligand. Kit contains Ir-catalyst and Diazirine PEG3 Biotin to perform 10 proteomics experiments. To link to an acid on a small molecule, the Dione Kit is available.
Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
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문서
In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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