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Merck
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FSUPMMRO

Roche

FastStart Universal Probe Master (Rox)

sufficient for 250 reactions, sufficient for 1250 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

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모든 사진(2)

About This Item

UNSPSC 코드:
41106300
NACRES:
NA.55

사용

sufficient for 1250 reactions
 sufficient for 250 reactions
 sufficient for 5000 reactions

Quality Level

100

특징

dNTPs included: no
hotstart

제조업체/상표

Roche

포장

pkg of 1250 x 20 μL reactions (04913957001)
pkg of 250 x 20 μL reactions (04913949001)
pkg of 5000 x 20 μL reactions (04914058001)

기술

RT-qPCR: suitable
qPCR: suitable

입력

purified DNA

검출 방법

probe-based

관련 카테고리

일반 설명

Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart™ Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. FastStart™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a preincubation step at +95°C).
Universal ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.

FastStart™ Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX, without modification or adjustments to the specific instrument or protocol. This ready-to-use, 2x concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format. FastStart™ Universal Probe Master (Rox) generates excellent results on instruments such as the Applied Biosystems 7900 HT Fast Real-Time PCR System or the Applied Biosystems 7500 Real-Time PCR System. This product is not intended for use with the LightCycler® Instruments.

애플리케이션

FastStart™ Universal Probe Master (Rox) has been used:
  • in TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR) reactions for the quantification of endogenous miRNAs, such as MIR376B, MIR376A and MIR181A
  • in reverse transcriptase (RT-PCR) to study tumor necrosis factor (TNF) expression in whole synovial tissue of undifferentiated peripheral inflammatory arthritis (UPIA) patients
  • for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich by quantitativePCR
Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.

특징 및 장점

  • Increase qPCR sensitivity and specificity.
Produce lower cycle threshold (Ct) values.

  • Use the master mix with any probe-based assay.
Achieve sensitive, specific results in assays with the Universal ProbeLibrary Probes or any other hydrolysis probe.

  • Amplify and detect a broad range of DNA or cDNA targets.
Amplify fragments up to 500 bp long, including those that are GC- or AT-rich.

  • Visualize amplification products on agarose gels.

  • Use robotic pipetting stations to set up qPCR reactions.
Use a master mix that is stable at room temperature during extended reaction setup times.

  • Prevent false positives resulting from carryover contamination.
Use this dUTP-containing mix with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions.

성분

FastStart Universal Probe Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and a reference dye.

품질

Function test: Each lot is tested for performance in qPCR using three templates: a GC–rich template, an AT-rich template, and a long template (approximately 440 bp).

기타 정보

For life science research only. Not for use in diagnostic procedures

법적 정보

FastStart is a trademark of Roche
LightCycler is a registered trademark of Roche
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Stefano Alivernini et al.
Frontiers in medicine, 5, 186-186 (2018-07-19)
Objectives: To examine synovial tissue (ST) predictors of clinical differentiation in patients with seronegative undifferentiated peripheral inflammatory arthritis (UPIA). Methods: Fourty-two patients with IgA/IgM-Rheumatoid Factor and anti-citrullinated peptide antibodies negative UPIA, naive to Disease-Modifying Anti-Rheumatic Drugs, underwent Gray Scale (GSUS)
The essential function for serum response factor in T-cell development reflects its specific coupling to extracellular signal-regulated kinase signaling.
Mylona A, et al.
Molecular and Cellular Biology, 31(2), 267-276 (2011)
Matthew L Hillestad et al.
Human gene therapy, 23(10), 1116-1126 (2012-07-28)
Reporter genes are important tools for assessing vector pharmacology in vivo. Although useful, current systems are limited by (1) the need to generate a new vector for each different reporter, (2) the inability to package reporter genes in small vectors
Kumsal Ayse Tekirdag et al.
Autophagy, 9(3), 374-385 (2013-01-17)
Macroautophagy (autophagy herein) is a cellular catabolic mechanism activated in response to stress conditions including starvation, hypoxia and misfolded protein accumulation. Abnormalities in autophagy were associated with pathologies including cancer and neurodegenerative diseases. Hence, elucidation of the signaling pathways controlling
Gozde Korkmaz et al.
PloS one, 8(12), e82556-e82556 (2013-12-21)
Autophagy is a vesicular trafficking process responsible for the degradation of long-lived, misfolded or abnormal proteins, as well as damaged or surplus organelles. Abnormalities of the autophagic activity may result in the accumulation of protein aggregates, organelle dysfunction, and autophagy
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문서

핫스타트 PCR

핫스타트(Hot Start) PCR의 목적은 비특이적 증폭을 줄이고 프라이머 이량체의 형성을 방지하며 제품 수율을 높이기 위해 PCR 반응을 억제하는 것입니다.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

관련 콘텐츠

PCR 애플리케이션

중합효소연쇄반응(PCR)은 핵산 분자를 증폭하는 기술이며 RT-PCR, hot start PCR, end point PCR을 포함한 여러 애플리케이션에서 일반적으로 활용됩니다.

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

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