LC/MS Analysis of Sorbitol and Mannitol on SeQuant^™ ZIC-HILIC

column

SeQuant ZIC-cHILIC, PEEK, 250 x 4.6 mm, 3 μm, 100Å (1.50662.0001)

mobile phase

[A] acetonitrile; [B] 0.1% ortho-phosphoric acid in Milli-Q^® water; (90:10, A:B)

flow rate

1.5 mL/min

pressure

1682 psi (116 bar)

column temp.

45 °C (oven), 40 °C (detector cell)

detector

Refractive Index (RI) (cell: 10 μL)

injection

20 μL

sample

5.0 mg/mL (5000 ppm) each of sorbitol and mannitol in diluent. (Diluent: Acetonitrile: 0.1% ortho-phosphoric acid in Milli-Q^® water 75:25 (v/v))