Human retinoblastoma cell line derived from a female Caucasian. The cells are semi-adherent, some neuronal differentiation can occur when subculturing. Tumourigenic in rabbits.
Split subconfluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO2; 37°C. Saturation density: 2 x 1000,000 cells/ml. Cells are semi-adherent. Scrape cells from surface; do not use trypsin.
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