540020

Pluripotin

• Synonym(s): Pluripotin, Ras GTPase-Activating Protein Inhibitor II, SC1, N-(3-(7-(1,3-dimethyl-1H-pyrazol-5-ylamino)-1-methyl-2-oxo-1,2-dihydropyrimido[4,5-d]pyrimidin-3(4H)-yl)-4-methylphenyl)-3-(trifluoromethyl)benzamide, ERK Inhibitor VII
• Empirical Formula (Hill Notation): C₂₇H₂₅F₃N₈O₂
• Molecular Weight: 550.54
• UNSPSC Code: 12352200

Properties

• Assay: ≥97% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 50 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable dihydropyrimido-pyrimidine compound that effectively upregulates GTP-bound active Ras level and inhibits LIF- (leukemia inhibitory factor) induced Erk1/2 phosphorylation in OG2-mES (murine Embryonic Stem) cells (1 µM) via direct interaction with Erk and RasGAP (K_D = 212 and 98 nM in binding RasGAP 729-1042 catalytic domain and Erk1, respectively), without affecting cellular STAT3 or GSK-3β/Wnt signaling. Pluripotin/SC1 is shown to greatly complement and enhance the efficiency of LIF in deriving ESCs from blastocysts of several murine strains and effectively maintain the pluripotent state of the ESCs even after 11 passages in cultures. SC1-expanded ESCs effectively form EBs (embryonic bodies) in vitro upon SC1 and LIF withdrawal and are capable of generating chimeric offsprings from surrogate mice when injected into blastocysts from different strains in vivo.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Yang, W., et al. 2009. Stem Cells27, 383.
Chen, S., et al. 2006. Proc. Natl. Acad. Sci. USA103, 17266.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable