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  • MALDI-TOF to compare polysaccharide profiles from commercial health supplements of different mushroom species.

MALDI-TOF to compare polysaccharide profiles from commercial health supplements of different mushroom species.

Food chemistry (2016-01-19)
Marta López-García, María Sonia Dopico García, José Manuel López Vilariño, María Victoria González Rodríguez
ABSTRACT

In this work MALDI-TOF mass spectroscopy was investigated to characterise the β-glucan profiles of several commercial health supplements, without any derivatisation or purification pre-treatment. The effect of two solvents (water and dimethyl sulfoxide) and two MALDI matrices (2,5-dihydroxybenzoic acid and 2',4',6'-trihydroxyacetophenone) was first evaluated on dextran standards. MALDI-TOF was found as a useful and quick technique to obtain structural information of diverse food supplements based on mushroom extracts. The MALDI polysaccharide profiles of 5 supplements from different mushroom species were qualitatively similar showing [Glucan+Na](+) cations with a peak-to-peak mass difference of 16 Da consistent with the repeating unit of the β-(1→3)-glucan. The profiles strongly depended on the sample solvent used, with m/z values around 5000-8000 for water and 2000 for dimethyl sulfoxide; differences between samples were revealed in the molecular weight of the aqueous preparation, with the highest values for Maitake and Cordyceps species.

MATERIALS
Product Number
Brand
Product Description

General Purpose Viscosity Standard; UKAS ISO/IEC17025 and ISO 17034 certified, viscosity 5738 mPa.s (25 °C)
General Purpose Viscosity Standard; UKAS ISO/IEC17025 and ISO 17034 certified, viscosity 990.0 mPa.s (25 °C)