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  • Silyl protecting groups for oligonucleotide synthesis removed by a ZnBr2 treatment.

Silyl protecting groups for oligonucleotide synthesis removed by a ZnBr2 treatment.

Nucleosides, nucleotides & nucleic acids (2005-10-27)
Fernando Ferreira, François Morvan
ABSTRACT

An oligonucleotide protected with N-(trimethylsilyloxycarbonyl) (Teoc) and P-(trimethylsilylethanol) (Tse) groups was synthesized and deprotected by a single ZnBr2 treatment. Finally it was released from the solid support by cleavage of a disulfide linkage with TCEP. The olgonucleotide was obtained without any basic treatment.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Zinc bromide, 99.999% trace metals basis
Sigma-Aldrich
Zinc bromide, AnhydroBeads, −10 mesh, 99.999% trace metals basis