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  • Analysis of diltiazem and desacetyldiltiazem in serum by high-performance liquid chromatography.

Analysis of diltiazem and desacetyldiltiazem in serum by high-performance liquid chromatography.

Polish journal of pharmacology (1995-09-01)
D Paczkowski, R Dabrowski, D Sitkiewicz, Z Sadowski
ABSTRACT

This paper describes a simple high-performance liquid chromatographic (HPLC) method for the determination of diltiazem and desacetyldiltiazem in human serum. After basic methyl-tert-butyl ether extraction and back-extraction with hydrochloric acid, the drug and its metabolite was injected into a Supelcosil LC-CN column and the absorbance of the eluate was measured at 240 nm. The sensitivity was 5 ng/ml and the obtained precision, selectivity and stability during storage were adequate for the performed clinical studies in patients therapeutically treated with diltiazem.

MATERIALS
Product Number
Brand
Product Description

Supelco
SUPELCOSIL LC-CN HPLC Column, 3 μm particle size, L × I.D. 7.5 cm × 4.6 mm
Supelco
SUPELCOSIL LC-CN Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
SUPELCOSIL LC-CN HPLC Column, 3 μm particle size, L × I.D. 3.3 cm × 3 mm
Supelco
SUPELCOSIL LC-CN HPLC Column, 3 μm particle size, L × I.D. 3.3 cm × 4.6 mm
Supelco
SUPELCOSIL LC-CN Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
SUPELCOSIL LC-CN HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
SUPELCOSIL LC-CN HPLC Column, 3 μm particle size, L × I.D. 7.5 cm × 3 mm
Supelco
SUPELCOSIL LC-CN HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
SUPELCOSIL LC-NH2-NP Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
SUPELCOSIL LC-NH2-NP HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm