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R6625

Sigma-Aldrich

Ribonucleic acid from torula yeast

Type VI

Synonym(s):

RNA

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.51

type

Type VI

storage temp.

−20°C

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General description

Ribonucleic Acid (RNA) from torula yeast is the substrate for the RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).

Application

Ribonucleic Acid (RNA) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.

Ribonucleic acid (RNA) from torula yeast may be used as a substrate for studying ribonuclease activities of enzymes such as ribonuclease-A, ribonuclease T1 (RNAase), and bougainvillea xbuttiana antiviral protein 1 (BBAP1)

Quality

Phosphorus: 8.0-10.0% (anhydrous)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
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