MAK118
Invertase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
β-fructofuranosidase Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
application(s)
cosmetics
environmental
food and beverages
detection method
colorimetric
fluorometric
storage temp.
−20°C
General description
Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.
Application
Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.
Principle
In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.
Storage Class Code
10 - Combustible liquids
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Khaled M Hazzouri et al.
Nature communications, 10(1), 4680-4680 (2019-10-17)
Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa. Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species. Here we report
Roua Gabriela Popescu et al.
Animals : an open access journal from MDPI, 10(5) (2020-05-14)
The purpose of this study was to examine the effects of dietary inclusion of two additives at the final concentration of 0.5% bilberry (E1) and 1% walnut (E2) leaves powder in the basal diet on digestive health of hens. A
Domenica Farci et al.
Journal of experimental botany, 67(11), 3303-3312 (2016-04-17)
Invertases are a widespread group of enzymes that catalyse the conversion of sucrose into fructose and glucose. Plants invertases and their substrates are essential factors that play an active role in primary metabolism and in cellular differentiation and by these
Mikael Ulfstedt et al.
Frontiers in plant science, 9, 1887-1887 (2019-01-09)
Hexokinases is a family of proteins that is found in all eukaryotes. Hexokinases play key roles in the primary carbon metabolism, where they catalyze the phosphorylation of glucose and fructose, but they have also been shown to be involved in
Identification and Functional Characterization of Sugarcane Invertase Inhibitor (ShINH1): A Potential Candidate for Reducing Pre-and Post-harvest Loss of Sucrose in Sugarcane.
Shivalingamurthy S G, et al.
Frontiers in Plant Science, 9 (2018)
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