SCR006

Accumax^™Cell Counting Solution in DPBS Sterile-filtered Cell Culture Tested

A cell detachment solution of proteolytic, collagenolytic & DNAse enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

• Synonym(s): Accumax Cell Solution, Cell Counting Solution
• UNSPSC Code: 12352204
• eCl@ss: 32160801
• NACRES: NA.78

Properties

• Quality Level: 300
• form: liquid
• manufacturer/tradename: Chemicon^®
• input: sample type mesenchymal stem cell(s); sample type: mouse embryonic stem cell(s); sample type epithelial cells; sample type cardiac stem cell(s); sample type: human embryonic stem cell(s); sample type pancreatic stem cell(s); sample type hematopoietic stem cell(s); sample type induced pluripotent stem cell(s); sample type neural stem cell(s)
• shipped in: dry ice

Description

General description

A cell detachment solution of proteolytic, collagenolytic and DNAse enzymes. Useful on a variety of cells lines however not all cells or cell types are affected by ACCUMAX^™, also passage number and confluency can influence how well the reagent does because the amounts and makeup of the collagen extracellular matrix will vary with age and culture state. Because ACCUMAX is a more gentle, less univerally proteolytic cleavage mixture reagent amounts and incubation times need to be optimized for each cell system.

The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic. ACCUMAX does not contain mammalian or bacterial derived products.

Each lot of ACCUMAX is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.



ACCUMAX has been used successfully on numerous cell types including: CHO, BHK, 293, COS, Sf9, and catfish primary hepatocyte cells.

Application

A cell detachment solution of proteolytic, collagenolytic & DNAse enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

Physical form

100 ml, ready to use, frozen sterile liquid. 1X ACCUMAX^™ enzymes in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5mM EDTA-4Na.

Storage and Stability

Stable when stored at -20°C. Refer to lot expiration date. Recommended storage upon receipt is -20°C. After thawing, ACCUMAX may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMP. Use sterile technique when handling.

Legal Information

Accumax is a trademark of Innovative Cell Technologies, Inc.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable