5.08313

HSP90α Inhibitor, PU-29F

• Synonym(s): HSP90α Inhibitor, PU-29F, 2-Fluoro-9-(2-isopropoxyethyl)-8-(3,4,5-trimethoxybenzyl)-9H-purin-6-amine
• Empirical Formula (Hill Notation): C₂₀H₂₆FN₅O₄
• CAS Number: 422508-27-8
• Molecular Weight: 419.45
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Assay: ≥98% (HPLC)
• Quality Level: 100
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: off-white
• solubility: DMSO: 100 mg/mL
• storage temp.: 2-8°C

Description

General description

A cell-permeable 6-amino-purine based compound that selectively binds to HSP90a (EC₅₀ = 5.4 µM) and inhibits its activity in SKBr3 and MCF-7 breast cancer cells. Exhibits much reduced activity towards other paralogs (EC₅₀ = 22.85, 71, and 173 5.4 µM for HSP90b, GRP94 AND Trap-1, respectively). Selectively depletes cytosolic HER2 and induces its redistribution towards lysosomes and early endosomes (~20 5.4 µM) without affecting membrane associated HER2. Does not appear to be toxic towards nonmalignant cells.

A cell-permeable 6-amino-purine based compound that selectively binds to HSP90a (EC₅₀ = 5.4 µM) and inhibits its activity in SKBr3 and MCF-7 breast cancer cells. Exhibits much reduced activity towards other paralogs (EC₅₀ = 22.85, 71, and 173 5.4 µM for HSP90b, GRP94 AND Trap-1, respectively). Selectively depletes cytosolic HER2 and induces its redistribution towards lysosomes and early endosomes (~20 5.4 µM) without affecting membrane associated HER2. Does not appear to be toxic towards nonmalignant cells.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
HSP90a

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Patel, P.D., et al. 2013. Nat. Chem. Biol.9, 677.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable