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1.50960

Supelco

LiChrospher® 100 DIOL (5 µm) HPLC Columns

L x I.D. 4 mm x 4 mm LiChroCART™ HPLC guard-cartridge

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

LiChrospher® 100 DIOL (5 µm) LiChroCART™ 4-4, suitable for HPLC

material

stainless steel column

Quality Level

100

Agency

suitable for USP L20

product line

LiChrospher®

feature

endcapped: no

packaging

10 pak of

extent of labeling

8% Carbon loading

parameter

250 bar pressure
60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)

column L × I.D.

0.4 cm × 4 mm

surface area

350 m2/g

matrix

fully porous particle

matrix active group

Diol bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

storage temp.

no temp limit

General description

HPLC guard column
LiChrospher® DIOL has polar and hydrophobic properties, thus can be used as less polar alternative to LiChrospher Si 60 in normal phase applications or as less hydrophobic alternative to LiChrospher RP-8 in reversed phase applications. LiChrospher DIOL is also suitable for exclusion chromatography. This sorbent is packed into LiChroCART 4-4 HPLC guard columns, to be used in connection with LiChroCART HPLC cartridges, packed with LiChrospher DIOL, 5µm. The connection is being accomplished by use of the manu-CART NT cartrige holder (1.51486)

Application


  • Sensitive determination of a glyoxal-DNA adduct biomarker candidate by column switching capillary liquid chromatography electrospray ionization mass spectrometry.: This study describes the use of LiChrospher® 100 DIOL (5 µm) in column switching capillary liquid chromatography to determine glyoxal-DNA adducts, which are potential biomarkers for DNA damage. The method combines high sensitivity with electrospray ionization mass spectrometry for precise analysis, highlighting its application in biomarker research and analytical chemistry (Olsen et al., 2008).

Analysis Note

Selectivity: passes test

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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