14-628-M

TBK1 Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant, full length, human TBK1, for use in Kinase Assays.

• Synonym(s): NAK, NF-kappa-B-activating kinase, T2K, TANK-binding kinase-1
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• shelf life: 6 mo.
• mol wt: M_w 85.7 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_013254.2
• UniProt accession no.: Q9UHD2
• storage temp.: −70°C
• Gene Information: human ... TBK1(29110)

Description

General description

Research area: Cancer
Protein Target: TBK1, Target Sub-Family: Misc

TANK-binding kinase-1 (TBK1), also known as NF-κB activating kinase (NAK), is a serine-threonine kinase from the IκB kinase (IKK) family. It is encoded by tbk1 gene and is ubiquitously expressed in various tissues.

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

TANK-binding kinase-1 (TBK1) is mainly involved in activating interferon regulatory transcription factor 3/7 (IRF 3/7) and nuclear factor (NF-κB) signaling pathways, which are essential for regulating the production of type I interferons (IFN), including IFN-α and IFN-β and the activation of innate immunity. In addition, TBK1 also mediates various other cellular processes including autophagy, proliferation, immune response/inflammation survival, insulin signaling, and metabolism. TBK1 plays a significant role in cancer pathogenesis. In cancer cells, it enhances tumor growth by activating proliferation and survival signaling, promoting tumor-promoting cytokine production, and facilitating the secretion of immunosuppressive inflammatory cytokines. Overexpression of tbk1 gene is observed in various cancers including non-small cell lung cancer (NSCLC), pancreatic ductal adenocarcinoma (PDA), cholangiocarcinoma, clear cell renal cell carcinoma (ccRCC), adult T-cell leukemia, melanoma, esophageal cancer, and breast cancer.

Quality

routinely evaluated by phosphorylation of CK1tide (12-529)

Physical form

Ni²⁺/NTA-agarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.