05-1248

Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306

clone CMA306, from mouse

• Synonym(s): H3K9me1, Histone H3 (mono methyl K9), H3-K9-HMTase 1, Histone H3-K9 methyltransferase 1, Lysine N-methyltransferase 1A, Position-effect variegation 3-9 homolog, Su(var)3-9 homolog 1, Suppressor of variegation 3-9 homolog 1, histone-lysine N-methyltransfe
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CMA306, monoclonal
• species reactivity: human
• technique(s): inhibition assay: suitable (peptide); western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NP_003484
• UniProt accession no.: O43463
• shipped in: wet ice
• target post-translational modification: monomethylation (Lys9)
• Gene Information: human ... H3C1(8350)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

This antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3.

Immunogen

Epitope: monomethylated H3 Lys9

KLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3.

Application

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 05-1248, Anti-monomethyl H3 (Lys9) at 2ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.

Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 (Mouse Monoclonal Antibody) validated in WB, PIA to detect monomethyl Histone H3 (Lys9) also known as H3K9me1.

Quality

Evaluated by Western Blot on recombinant Histone H3 and HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract.

Target description

~17 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2a in PBS with 0.05% sodium azide.

Storage and Stability

Stable at 2-8°C for 1 year from date of receipt.

Analysis Note

Control
Recombinant Histone H3 and HeLa acid extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable