Quantitative determination of linoleic acid in infant formulas.

Quantitative determination of linoleic acid in infant formulas.

International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition (1991-01-01)

G Bellomonte, S Giammarioli, R Terilli

PMID1856052

ABSTRACT

The aim of this paper is to develop a gas chromatographic method for the quantitative determination of linoleic acid in adapted, preterm, follow-up and special infant formulas. For sample preparation, lipids were extracted with a Soxhlet extractor after acid hydrolysis, and methyl esters of fatty acids were prepared in a sealed vial with hydrochloric acid in methyl alcohol and analysed using a packed polar column (SP 2330). Triheptadecanoin was chosen as internal standard. The method yielded reliable and repeatable results for all the examined formulas.

MATERIALS

Product Number

Brand

Product Description

24074

Supelco

SP^®-2330 Capillary GC Column, L × I.D. 60 m × 0.32 mm, d_f 0.20 μm

24020-U

Supelco

SP^®-2330 Capillary GC Column, L × I.D. 60 m × 0.25 mm, d_f 0.20 μm

24019

Supelco

SP^®-2330 Capillary GC Column, L × I.D. 30 m × 0.25 mm, d_f 0.20 μm

24073

Supelco

SP^®-2330 Capillary GC Column, L × I.D. 30 m × 0.32 mm, d_f 0.20 μm

24018

Supelco

SP^®-2330 Capillary GC Column, L × I.D. 15 m × 0.25 mm, d_f 0.20 μm