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  • Sensitive and specific phenotypic assay for metallo-beta-lactamase detection in Enterobacteria by use of a moxalactam disk supplemented with EDTA.

Sensitive and specific phenotypic assay for metallo-beta-lactamase detection in Enterobacteria by use of a moxalactam disk supplemented with EDTA.

Journal of clinical microbiology (2011-05-06)
Emilie Pluquet, Guillaume Arlet, Edouard Bingen, Laurence Drieux, Hedi Mammeri
ABSTRACT

Moxalactam is highly hydrolyzed by plasmid-mediated metallo-β-lactamases (MBLs), whereas it is poorly inactivated by serine-active carbapenemases. This study demonstrated that moxalactam resistance constituted an effective screen for MBL expression in enterobacteria, which could be confirmed, even in low-MBL-producing isolates, by a disk potentiation test using moxalactam and EDTA.

MATERIALS
Product Number
Brand
Product Description

Millipore
Moxalactam Supplement, suitable for microbiology