- High-speed counter-current chromatographic isolation of ricinine, an insecticide from Ricinus communis.
High-speed counter-current chromatographic isolation of ricinine, an insecticide from Ricinus communis.
Journal of chromatography. A (2009-03-03)
Cristiane de Melo Cazal, Jaqueline Raquel Batalhão, Vanessa de Cássia Domingues, Odair Corrêa Bueno, Edson Rodrigues Filho, Moacir R Forim, Maria Fátima G Fernandes da Silva, Paulo Cezar Vieira, João Batista Fernandes
PMID19249053
ABSTRACT
The alkaloid ricinine, an insecticide for leaf-cutting ant (Atta sexdens rubropilosa), was obtained from Ricinus communis. A two-phase solvent system composed of CH(2)Cl(2)/EtOH/H(2)O (93:35:72, v/v/v) was used for high-speed counter-current chromatographic (HSCCC) isolation of ricinine in high yield and with over 96% purity, as determined by liquid and gas chromatography-mass spectrometry (LC-MS and GC-MS). Identification of ricinine was performed by comparison of (1)H NMR, (13)C NMR and LC-MS/MS data.
MATERIALS