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G005891

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis® Express C18, 25 cm x 4.6 mm I.D., 5 μm particles (50538-U)
mobile phase: [A] acetonitrile; [B] methanol; (95:5, A:B)
flow rate: 1.5 mL/min
pressure: 1726 psi (119 bar)
column temp.: 35 °C
detector: UV, 265 nm
injection: 10 μL
sample: 50 μg/mL in 90:10, acetonitrile:methanol

suitability

application for HPLC

application(s)

clinical
vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of the Ascentis Express C18, 5 μm, for the routine analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis Express C18, 5 μm are presented.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Ergocalciferol, 40,000,000 USP units/g
    Cholecalciferol, ≥98% (HPLC)

analytical column

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