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M0404

Sigma-Aldrich

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture, with Gamborg′s vitamins

Synonym(s):

MS0 Medium, MSO Medium, Murashige and Skoog (Gamborg Modified) Basal Media, MS Basal Medium

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About This Item

MDL number:
MFCD00240976
UNSPSC Code:
12352207
NACRES:
NA.72

Quality Level

200

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

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Application

Murashige and Skoog Basal Medium has been used:
  • to germinate the seeds for seedling growth and germination studies
  • in the suspension culture of Nicotiana plumbaginifolia for transformation
  • in the root culture of hairy clone A. artemisiifolia T4

Formula variant

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Pictograms

Flame over circleExclamation mark
GHS03,GHS07

Signal Word

Warning

Hazard Statements

H272,H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana
Gilson PR, et al.
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Ten Hyptis suaveolens hairy root lines were established by infecting nodal explants with K599+pGus-GFP+ and ATCC15834+pTDT strains from Agrobacterium rhizogenes. Genetic transformation was confirmed by epifluorescence and plagiotropic hairy root growth in absence of growth regulators. Cytotoxicity was determined using
Ken-Der Wang et al.
The Plant cell, 32(1), 166-185 (2019-11-07)
Multiple long-distance signals have been identified for pathogen-induced systemic acquired resistance, but mobile signals for symbiont-induced systemic resistance (ISR) are less well understood. We used ISR-positive and -negative mutants of maize (Zea mays) and the beneficial fungus Trichoderma virens and
Overexpression of 20-oxidase confers a gibberellin-overproduction phenotype in Arabidopsis
Huang S, et al.
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Two modes of plant immunity against biotrophic pathogens, Effector Triggered Immunity (ETI) and Pattern-Triggered Immunity (PTI), are triggered by recognition of pathogen effectors and Microbe-Associated Molecular Patterns (MAMPs), respectively. Although the jasmonic acid (JA)/ethylene (ET) and salicylic acid (SA) signaling
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