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A2050

Sigma-Aldrich

Avidin–FITC from egg white

buffered aqueous solution

Synonym(s):

Avidin–Fluorescein isothiocyanate

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About This Item

MDL number:
MFCD00130585
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

FITC conjugate

form

buffered aqueous solution

extent of labeling

3-5.5 mol fluorochrome per mol protein

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL
indirect immunofluorescence: 1:200

UniProt accession no.

P02701

shipped in

dry ice

storage temp.

−20°C

Gene Information

chicken ... AVD(396260)

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General description

Avidin is homotetrameric protein (68 kDa) that is obtained from egg whites.

Application

Avidin-FITC from egg white has been used in:
  • flow cytometry
  • double immunostaining
  • double immunofluorescence
  • immunnohistochemistry

To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.

Physical form

Solution in 0.01 M phosphate buffered saline containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide.

Preparation Note

Affinity purified protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mohamed A Abouelkhair et al.
PloS one, 13(9), e0204450-e0204450 (2018-09-28)
Bacterial infections from Staphylococcus pseudintermedius are the most common cause of skin infections (pyoderma) affecting dogs. Two component pore-forming leukocidins are a family of potent toxins secreted by staphylococci and consist of S (slow) and F (fast) components. They impair
L Struthers et al.
Analytical biochemistry, 255(1), 20-31 (1998-02-04)
8-Oxodeoxyguanosine is present in DNA from many tissues. The direct demonstration of 8-oxodeoxyguanosine as a potential biomarker of oxidative DNA damage has implications for the study of mutagenesis, carcinogenesis, and free radical toxicity. Avidin is shown here to bind with
The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis
Jain A and Cheng K
Journal of Controlled Release : Official Journal of the Controlled Release Society, 245, 27-40 (2017)
Stephanie G Grancharov et al.
The journal of physical chemistry. B, 109(26), 13030-13035 (2006-07-21)
Monodisperse magnetic nanoparticles (NPs) could enable the ultra-sensitive magnetic detection of biological analytes. However, rendering these particles biocompatible has remained a challenge. We report the bio-functionalization and detection of 12-nm manganese ferrite NPs. We have achieved the site-specific binding of
Linlin Sun et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(33), 6595-6607 (2019-06-12)
Expressional changes of pain-associated genes in primary sensory neurons of DRG are critical for neuropathic pain genesis. DNA methyltransferase (DNMT)-triggered DNA methylation silences gene expression. We show here that DNMT1, a canonical maintenance methyltransferase, acts as the de novo DNMT
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