Skip to Content
Merck
  • Simultaneous determination of sixteen amide fungicides in vegetables and fruits by dispersive solid phase extraction and liquid chromatography-tandem mass spectrometry.

Simultaneous determination of sixteen amide fungicides in vegetables and fruits by dispersive solid phase extraction and liquid chromatography-tandem mass spectrometry.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2015-03-24)
Yin-Liang Wu, Ruo-Xia Chen, Yong Zhu, Jian Zhao, Ting Yang
ABSTRACT

A modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method using multi-walled carbon nanotubes (MWCNTs) as a reversed-dispersive solid phase extraction (r-dSPE) material combined with ultra-high liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed for the simultaneous determination of 16 novel amide fungicides in vegetables and fruits. After extraction with acetonitrile, a dSPE cleanup procedure, which was developed after the optimization of the type and amount of MWCNTs, the pH value of the extract, the extraction time for MWCNTs, and the type of eluent with MWCNTs material, was conducted. The determination of the target compounds was conducted in less than 7.0 min while the specificity is ensured through the MRM acquisition mode. The linearity of the analytical response across the studied range of concentrations (0.25-500 μg/L) was excellent, obtaining correlation coefficients higher than 0.997. The samples were quantified with the matrix matched standard solutions. The average recoveries in cabbage, celery, strawberry, and grape at three spiked levels (0.01, 0.5, and 5.0mg/kg) were ranged from 72.4 to 98.5% with all RSDs lower than 10%. The limits of detection were below 0.003 mg/kg and the limits of quantification did not exceed 0.01 mg/kg in all matrices. The method demonstrated to be suitable for the simultaneous determination of 16 novel amide fungicides in vegetables and fruits.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cyclohexane, Laboratory Reagent, ≥99.8%
Sigma-Aldrich
Sodium hydroxide, puriss. p.a., ACS reagent, reag. Ph. Eur., K ≤0.02%, ≥98%, pellets
Sigma-Aldrich
Sodium hydroxide, ACS reagent, ≥97.0%, pellets
Sigma-Aldrich
Sodium hydroxide, reagent grade, 97%, flakes
Sigma-Aldrich
Sodium hydroxide solution, 50% in H2O
Sigma-Aldrich
Methanol, BioReagent, ≥99.93%
Sigma-Aldrich
Cyclohexane, puriss. p.a., ACS reagent, ≥99.5% (GC)
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Sodium hydroxide, pellets, semiconductor grade, 99.99% trace metals basis
Sigma-Aldrich
Sodium hydroxide, beads, 16-60 mesh, reagent grade, 97%
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%
Sigma-Aldrich
Cyclohexane, suitable for HPLC, ≥99.7%
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
Sigma-Aldrich
Methanol, suitable for HPLC, ≥99.9%
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Penthiopyrad, PESTANAL®, analytical standard
Supelco
Bixafen, PESTANAL®, analytical standard
Supelco
Sodium hydroxide concentrate, 0.1 M NaOH in water (0.1N), Eluent concentrate for IC
Supelco
Fenoxanil, PESTANAL®, analytical standard
Sigma-Aldrich
Methanol, NMR reference standard
Supelco
Methanol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Sodium hydroxide, ultra dry, powder or crystals, 99.99% trace metals basis
Sigma-Aldrich
Sodium hydroxide solution, 1.0 N, BioReagent, suitable for cell culture
Supelco
Cyclohexane, analytical standard
Supelco
Methanol, analytical standard
Supelco
Sodium hydroxide solution, 49-51% in water, eluent for IC
Sigma-Aldrich
Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H2O
Sigma-Aldrich
Sodium hydroxide, BioUltra, for luminescence, ≥98.0% (T), pellets
Sigma-Aldrich
Methanol, anhydrous, 99.8%