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N1905

Sigma-Aldrich

Neurotrophin-3 human

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

Synonym(s):

HDNF, NTF3, NT-3

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

1-40 ng/mL EC50

packaging

pkg of 5 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... NTF3(4908)

General description

NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family. This protein is made up of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.

Application

Neurotrophin-3 human has been used in the magnetic sorting of oligodendrocyte progenitor cells.

Biochem/physiol Actions

Neurotrophin-3 (13.6 kDa or 27.2 kDa dimer) is a member of the neurotrophin family. It has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. NT-3 is important in development and maintenance of neuronal populations and promotes differentiation of neural crest derived sensory and sympathetic neurons. It is critical for proprioceptive 1a afferent neurons, which relay information from peripheral muscle spindles to motoneurons, sending projections to spinocerebellar neurons. NT-3 is also critical in the superior cervical and nodose ganglia.

Physical form

Lyophilized from a 0.2 m filtered solution in phosphate buffered saline (PBS) containing 50 μg of bovine serum albumin (BSA) per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using a TrkB-transfected cell line BaF-TrkB-BD.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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