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I3786

Sigma-Aldrich

Protease Inhibitor Cocktail

Animal-Free, DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use with mammalian cell and tissue extract, DMSO solution

Synonym(s):

Protease inhibitor

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

product name

Protease Inhibitor Cocktail, Animal Component Free, for use with mammalian cell and tissue extract, DMSO solution

Quality Level

200

form

DMSO solution

storage temp.

−20°C

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General description

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts.

Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.

Application

Animal component free (ACF). Optimized and tested for general use. Can also be used for purification of histidine-tagged proteins, as chelating agents have been omitted.

Components

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Physical form

The components in this ACF protease inhibitor cocktail do not originate from animal sources and can thus be used in the production of animal-free proteins.
This ACF cocktail is supplied as a ready-to-use solution in DMSO. One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 108 cells per mL.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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