MAB1522
Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7
ascites fluid, clone CGA7, Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
clone
CGA7, monoclonal
species reactivity
human, rat, chicken, rabbit, monkey
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
shipped in
dry ice
target post-translational modification
unmodified
Specificity
Antibody recognizes actin isotypes alpha and gamma of smooth muscle cells, but not skeletal or cardiac muscle cells. Reactive with myoepithelium, but nonreactive with other mesenchymal and all epithelial cells. The antibody labels eiomyosarcomas and leiomyomas but does not react with other neoplasms such as carcinomas or lymphoma.
Immunogen
Chicken gizzard actin
Epitope: smooth muscle gamma & alpha actin
Application
Detect Actin using this Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7 validated for use in WB, IH, IH(P).
Western Blot: 1:1000 (approximately 43 kDa)
Immunohistochemistry (paraffin, formalin, deparaffinized methacarn-fixed tissue: 1:10,000 or alcohol fixation)
Optimal dilutions must be determined by the end user.
Immunohistochemistry (paraffin, formalin, deparaffinized methacarn-fixed tissue: 1:10,000 or alcohol fixation)
Optimal dilutions must be determined by the end user.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Cultured circular smooth muscle from the rabbit colon.
Kao, H W, et al.
In Vitro Cellular & Developmental Biology, 24, 787-794 (1988)
Xin Yao et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 13(1), 161-169 (2007-01-04)
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Functional expression of the GABAB receptor in human airway smooth muscle.
Osawa, Y; Xu, D; Sternberg, D; Sonett, JR; D'Armiento, J; Panettieri, RA; Emala, CW
American Journal of Physiology. Lung Cellular and Molecular Physiology null
Mary E Donohoe et al.
Molecular cell, 25(1), 43-56 (2007-01-16)
In mammals, inactivation of one X chromosome in the female equalizes gene dosages between XX females and XY males. Two noncoding loci, Tsix and Xite, together regulate X chromosome fate by controlling homologous chromosome pairing, counting, and mutually exclusive choice.
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