Validated Cell Types for the Scepter™ 3.0 Cell Counter

The Scepter™ counter has been shown to be accurate over a range of cell types and sizes. See below for a list of mammalian and other cell types that have been validated for Scepter™ counting.

Measured

• Recommended based on size
• Customer Validated
• MilliporeSigma Scepter™ 2.0 Validated
• MilliporeSigma Scepter™ 3.0 Validated

¹ histograms may be cut-off at high concentrations, dilution may be needed

Cell Type	size (μm)	40 μm sensor	60 μm sensor
2102 Ep	15-19		•
454 beads	28		•
A10	22 - 24		•
A172	15		•
A253	14-18		•
A375	16		•
A431	15-17		•
A549	11-15		•
Algae (various)	7-9	•	•
B35	13-16	•	•
B Cells	6-11	•
C2C12	12	•	•
C305	12-14	•	•
C6	12-13		•
CA46	10-12	•	•
Caco-2	17		••
CHO	13-17		••
COS-1	12	•
Cos-7	15		•
D283	12	•
Daudi	10-12	•	•
DU-145	15-17		•
EL4 (Blood Murine Lymphoblast)	12-14	•	•
Epithelia	14-15		•
Epithelia MDA-MB-231	16-17	•	•
HCC827 (Human lung adenocarcinoma)	18		•
HCT-116	10	•	•
HEK293	11-16		••
HeLa	12-15	•	••
HepG2	12-17	•1	••
HFF	18-20		•
Hs27	14	•	•
HT-1080 (fibrosarcoma)	14-16		••
HT-29	11		•
HUH7 (Hepatoma)	not available
Human ES Cells	9-12	•
HUVEC	14-15		•
Hybridoma	13-14		•
IMR-32	12-14	•	•
IMR-90	15	•	•
iPSCs	16-17	•1	•
Jurkat	12-13	•	••
K562	22		•
KB	14		•
KG-1	10-13	•	•
L6	14-16		•
LNCaP	15-16		•
Luminex®beads	5-6	•
MCF7	15-21		••
MDCK	13-15	•	••

Measured

• Recommended based on size
• Customer Validated
• MilliporeSigma Scepter™ 2.0 Validated
• MilliporeSigma Scepter™ 3.0 Validated

¹ histograms may be cut-off at high concentrations, dilution may be needed

Cell Type	size (μm)	40 μm sensor	60 μm sensor
Meg-01	16-17		•
MG-63	15-17		•
Mouse ES Cell	5-13	•	•
MN9D (Mouse Dopaminergic Neuronal)	15		•
MSc (Mesenchymal Stem)	15-16	••1	•
MRC-5	18		•
NCI-H146	10-13	•
NIH 3T3	15	•	••
NTERA2, clone D1	13	•	•
OK	17-18		•
PBMCs	7-12	•
PC12	9-15	•	•
Primary Astrocytes	7	•
Primary Neuronal Cell	Varies	•
Raji	12-15	•	•
Ramos	11-12	•	•
Rat Dorsal Root Ganglion Cells	20-30		•
Rat Whole Blood	4.6	••
Red Blood Cells	5-7	•
Rat Neural Stem Cell (RNSC)	11-13		•
RAW 264.7 Cell Line murine macrophage from blood	11-13	•	•
RBL	11-13	•	•
RIN-mF5	13-14		•
S462 (Human Peripheral Nerve Sheath Tumor)	16		•
SF9	13		•
SH-SY5Y	12		•
Sk-Br-3	15-20		•
SK-MEL-28	17-19		•
SK-N-MC	14-15		•
SK-N-SH	14-15		•
Splenocytes	7-9	•
SW-480	15		•
SW-620	13-14	•	•
T84	14-18		•
T98G	17		•
T cells: naïve	5-7		•
T cells: CD3+	11-12	•	•
THP 1 (Human blood monocyte)	12-13	•	•
TF-1	13-14	•	•
U251	16-20		•
U2OS	16-19		•
U266	12		•
U87 (Human Glioblastoma)	12-14		•
U937	11-13	•	•
WI-38	12-15		•
Y79	13-14		•
Yeast- Pichia Pastoris	5	•
Yeast - S. cerevisiae	6	•