HPLC Analysis of Mannose and Maltose Sugars on Ascentis® Express HILIC
材料
分析カラム
製品番号
詳細
価格
CONDITIONS
column
Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase
[A] water; [B] acetonitrile; (25:75, A:B)
flow rate
1.0 mL/min
column temp.
35 °C
detector
ELSD, 55 °C, 3.5 bar nitrogen
injection
10 μL
詳細
アナリシスノート
This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany