HPLCによるアミノ酸(極性、中性、酸性)の分析、Ascentis^® Si

column

Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0, with formic acid; [B] water; [C] acetonitrile

gradient

5% A constant; 10% B, 85% C to 16% B, 79% C in 20 min

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI (+), full scan

injection

2 μL

sample

as indicated, in 10:90, (50 mM ammonium formate/formic acid, (pH 3.0):acetonitrile