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  • Protein kinase CK2 is coassembled with small conductance Ca(2+)-activated K+ channels and regulates channel gating.

Protein kinase CK2 is coassembled with small conductance Ca(2+)-activated K+ channels and regulates channel gating.

Neuron (2004-09-15)
Wolfgang Bildl, Tim Strassmaier, Henrike Thurm, Jens Andersen, Silke Eble, Dominik Oliver, Marlies Knipper, Matthias Mann, Uwe Schulte, John P Adelman, Bernd Fakler
要旨

Small conductance Ca(2+)-activated K+ channels (SK channels) couple the membrane potential to fluctuations in intracellular Ca2+ concentration in many types of cells. SK channels are gated by Ca2+ ions via calmodulin that is constitutively bound to the intracellular C terminus of the channels and serves as the Ca2+ sensor. Here we show that, in addition, the cytoplasmic N and C termini of the channel protein form a polyprotein complex with the catalytic and regulatory subunits of protein kinase CK2 and protein phosphatase 2A. Within this complex, CK2 phosphorylates calmodulin at threonine 80, reducing by 5-fold the apparent Ca2+ sensitivity and accelerating channel deactivation. The results show that native SK channels are polyprotein complexes and demonstrate that the balance between kinase and phosphatase activities within the protein complex shapes the hyperpolarizing response mediated by SK channels.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
Anti-Casein Kinase 2 Antibody, Upstate®, from rabbit
Sigma-Aldrich
Anti-Potassium Channel SK2 antibody produced in rabbit, affinity isolated antibody, lyophilized powder