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Merck

Phytochemicals potently inhibit migration of metastatic breast cancer cells.

Integrative biology : quantitative biosciences from nano to macro (2015-06-30)
Stephanie Lemmo Ham, Samila Nasrollahi, Kush N Shah, Andrew Soltisz, Sailaja Paruchuri, Yang H Yun, Gary D Luker, Anupam Bishayee, Hossein Tavana
要旨

Cell migration is a major process that drives metastatic progression of cancers, the major cause of cancer death. Existing chemotherapeutic drugs have limited efficacy to prevent and/or treat metastasis, emphasizing the need for new treatments. We focus on triple negative breast cancer (TNBC), the subtype of breast cancer with worst prognosis and no standard chemotherapy protocols. Here we demonstrate that a group of natural compounds, known as phytochemicals, effectively block migration of metastatic TNBC cells. Using a novel cell micropatterning technology, we generate consistent migration niches in standard 96-well plates where each well contains a cell-excluded gap within a uniform monolayer of cells. Over time, cells migrate into and occupy the gap. Treating TNBC cells with non-toxic concentrations of phytochemicals significantly blocks motility of cells. Using a molecular analysis approach, we show that anti-migratory property of phytochemicals is partly due to their inhibitory effects on phosphorylation of ERK1/2. This study provides a framework for future studies to understand molecular targets of phytochemicals and evaluate their effectiveness in inhibiting metastasis in animal models of cancer.

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レスベラトロール, ≥99% (HPLC)
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L-リシン 一塩酸塩, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
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カルセイン AM, suitable for fluorescence, BioReagent, ≥90% (HPLC)
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カルセイン AM, Small Package (20 X 50 μg ), ≥95.0% (HPLC)
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L-リシン 一塩酸塩, reagent grade, ≥98% (HPLC)
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L-リシン 一塩酸塩, BioUltra, ≥99.5% (AT)
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カルセイン AM 溶液, 4 mM in DMSO, ≥90% (HPLC), solution
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L-リシン 一塩酸塩, SAJ special grade, ≥99.0%
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