コンテンツへスキップ
Merck
  • Highly magneto-responsive multilayer microcapsules for controlled release of insulin.

Highly magneto-responsive multilayer microcapsules for controlled release of insulin.

International journal of pharmaceutics (2014-08-28)
Chunli Zheng, Yafei Ding, Xiaoqing Liu, Yunkai Wu, Liang Ge
要旨

In this study, magneto-responsive polyelectrolyte multilayer microcapsules were successfully prepared by the formation of shell with biocompatible iron oxide nanoparticles (Fe₃O₄ NPs) and polyallylamine hydrochloride (PAH) by layer-by-layer (LbL) self-assembly technique. The self-assembled microcapsules were characterized by SEM, TEM and zeta-potential analyzer. According to the pH sensitivity of the microcapsule membrane permeability, insulin was encapsulated, with the encapsulation efficiency of 92.08±5.57%. The in vitro release behavior in an external alternating magnetic field indicated that once the magnetic field was applied, the drug release was greatly accelerated. In addition, according to the observed pulse release upon cyclic on-off operations of magnetic field, it could be assumed that the magneto-responsive microcapsules had an excellent "switching on" effect, which might be attributed to the rearrangement of shell structure caused by magnetic nanoparticles twisting and polyelectrolyte chains shaking, hence the increase of microcapsule membrane permeability and the enhancement of insulin release.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
炭酸ナトリウム, powder, ≥99.5%, ACS reagent
Sigma-Aldrich
炭酸ナトリウム, ACS reagent, anhydrous, ≥99.5%, powder or granules
Sigma-Aldrich
塩化ナトリウム, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
塩化ナトリウム 溶液, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
塩化ナトリウム 溶液, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
クエン酸, meets analytical specification of Ph. Eur., BP, USP, E330, anhydrous, 99.5-100.5% (based on anhydrous substance)
Sigma-Aldrich
塩化ナトリウム, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
炭酸ナトリウム, ACS reagent (primary standard), anhydrous, 99.95-100.05% dry basis
Sigma-Aldrich
炭酸ナトリウム, ReagentPlus®, ≥99.5%
Sigma-Aldrich
エチレンジアミン四酢酸 溶液, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
クエン酸, ACS reagent, ≥99.5%
Sigma-Aldrich
塩化鉄(II), 98%
Sigma-Aldrich
塩化ナトリウム, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
塩化ナトリウム, JIS special grade, ≥99.5%
Sigma-Aldrich
塩化ナトリウム 溶液, BioUltra, for molecular biology, ~5 M in H2O
Sigma-Aldrich
エチレンジアミン四酢酸, anhydrous, crystalline, BioReagent, suitable for cell culture
Sigma-Aldrich
炭酸ナトリウム, puriss., meets analytical specification of Ph. Eur., BP, NF, FCC, E500, anhydrous, 99.5-100.5% (calc. to the dried substance)
Sigma-Aldrich
エチレンジアミン四酢酸, 99.995% trace metals basis
Sigma-Aldrich
炭酸ナトリウム, anhydrous, powder, 99.999% trace metals basis
Sigma-Aldrich
塩化ナトリウム, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
エチレンジアミン四酢酸, ACS reagent, 99.4-100.6%, powder
Sigma-Aldrich
塩化ナトリウム, 99.999% trace metals basis
Sigma-Aldrich
クエン酸, 99%
Sigma-Aldrich
炭酸ナトリウム, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99.5%
Sigma-Aldrich
クエン酸, ≥99.5%, FCC, FG
Sigma-Aldrich
塩化ナトリウム 溶液, 5 M
Sigma-Aldrich
クエン酸, anhydrous, suitable for cell culture, suitable for plant cell culture
USP
クエン酸, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
エチレンジアミン四酢酸 二ナトリウム塩 溶液, BioUltra, for molecular biology, pH 8.0, ~0.5 M in H2O
Sigma-Aldrich
クエン酸, BioUltra, anhydrous, ≥99.5% (T)