- Metabolomics annotates ABHD3 as a physiologic regulator of medium-chain phospholipids.
Metabolomics annotates ABHD3 as a physiologic regulator of medium-chain phospholipids.
Nature chemical biology (2011-09-20)
Jonathan Z Long, Justin S Cisar, David Milliken, Sherry Niessen, Chu Wang, Sunia A Trauger, Gary Siuzdak, Benjamin F Cravatt
PMID21926997
要旨
All organisms, including humans, possess a huge number of uncharacterized enzymes. Here we describe a general cell-based screen for enzyme substrate discovery by untargeted metabolomics and its application to identify the protein α/β-hydrolase domain-containing 3 (ABHD3) as a lipase that selectively cleaves medium-chain and oxidatively truncated phospholipids. Abhd3(-/-) mice possess elevated myristoyl (C14)-phospholipids, including the bioactive lipid C14-lysophosphatidylcholine, confirming the physiological relevance of our substrate assignments.