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Merck
  • Cp*Rh-based indicator-displacement assays for the identification of amino sugars and aminoglycosides.

Cp*Rh-based indicator-displacement assays for the identification of amino sugars and aminoglycosides.

Chemistry (Weinheim an der Bergstrasse, Germany) (2006-03-08)
Friederike Zaubitzer, Andrey Buryak, Kay Severin
要旨

Indicator-displacement assays based on the organometallic complex [{Cp*RhCl2}2] (Cp*=pentamethylcyclopentadienyl) and the dye gallocyanine were used to sense amino sugars and aminoglycosides in buffered aqueous solution by conducting UV-visible spectroscopy. The data of three assays at pH 7.0, 8.0, and 9.0 were sufficient to distinguish between the amino sugars galactosamine, glucosamine, mannosamine and the aminoglycosides kanamycin A, kanamycin B, amikacin, apramycin, paromomycin, and streptomycin. Furthermore, the assays were used to characterize mixtures of aminoglycosides and obtain quantitative information about the respective analytes.

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製品内容

Sigma-Aldrich
Gallocyanine, Dye content 90 %