コンテンツへスキップ
Merck
  • WormFarm: a quantitative control and measurement device toward automated Caenorhabditis elegans aging analysis.

WormFarm: a quantitative control and measurement device toward automated Caenorhabditis elegans aging analysis.

Aging cell (2013-02-28)
Bo Xian, Jie Shen, Weiyang Chen, Na Sun, Nan Qiao, Dongqing Jiang, Tao Yu, Yongfan Men, Zhijun Han, Yuhong Pang, Matt Kaeberlein, Yanyi Huang, Jing-Dong J Han
要旨

Caenorhabditis elegans is a leading model organism for studying the basic mechanisms of aging. Progress has been limited, however, by the lack of an automated system for quantitative analysis of longevity and mean lifespan. To address this barrier, we developed 'WormFarm', an integrated microfluidic device for culturing nematodes. Cohorts of 30-50 animals are maintained throughout their lifespan in each of eight separate chambers on a single WormFarm polydimethylsiloxane chip. Design features allow for automated removal of progeny and efficient control of environmental conditions. In addition, we have developed computational algorithms for automated analysis of video footage to quantitate survival and other phenotypes, such as body size and motility. As proof-of-principle, we show here that WormFarm successfully recapitulates survival data obtained from a standard plate-based assay for both RNAi-mediated and dietary-induced changes in lifespan. Further, using a fluorescent reporter in conjunction with WormFarm, we report an age-associated decrease in fluorescent intensity of GFP in transgenic worms expressing GFP tagged with a mitochondrial import signal under the control of the myo-3 promoter. This marker may therefore serve as a useful biomarker of biological age and aging rate.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
シリコンオイル, viscosity 5 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, viscosity 10 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, viscosity 20 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, for oil baths (from −50°C to +200°C)
Sigma-Aldrich
シリコンオイル, viscosity 100 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, viscosity 1,000 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, viscosity 350 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, viscosity 500 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, viscosity 50 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, viscosity 10,000 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, viscosity 60,000 cSt (25 °C)
シリコンオイル, for melting point and boiling point apparatuses
Sigma-Aldrich
シリコンオイル, viscosity 100,000 cSt (25 °C)
Sigma-Aldrich
シリコンオイル, viscosity 30,000 cSt (25 °C)