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  • Covalent immobilization of catalase onto spacer-arm attached modified florisil: characterization and application to batch and plug-flow type reactor systems.

Covalent immobilization of catalase onto spacer-arm attached modified florisil: characterization and application to batch and plug-flow type reactor systems.

Enzyme and microbial technology (2011-12-07)
Ozlem Alptekin, S Seyhan Tükel, Deniz Yildirim, Dilek Alagöz
要旨

Catalase was covalently immobilized onto florisil via glutaraldehyde (GA) and glutaraldehyde+6-amino hexanoic acid (6-AHA) (as a spacer arm). Immobilizations of catalase onto modified supports were optimized to improve the efficiency of the overall immobilization procedures. The V(max) values of catalase immobilized via glutaraldehyde (CIG) and catalase immobilized via glutaraldehyde+6-amino hexanoic acid (CIG-6-AHA) were about 0.6 and 3.4% of free catalase, respectively. The usage of 6-AHA as a spacer arm caused about 40 folds increase in catalytic efficiency of CIG-6-AHA (8.3 × 10⁵ M⁻¹ s⁻¹) as compared to that of CIG (2.1 × 10⁴ M⁻¹ s⁻¹). CIG and CIG-6-AHA retained 67 and 35% of their initial activities at 5 °C and 71 and 18% of their initial activities, respectively at room temperature at the end of 6 days. Operational stabilities of CIG and CIG-6-AHA were investigated in batch and plug-flow type reactors. The highest total amount of decomposed hydrogen peroxide (TAD-H₂O₂) was determined as 219.5 μmol for CIG-6-AHA in plug-flow type reactor.

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製品内容

Sigma-Aldrich
フロリジル®, 100-200 mesh
カタログ番号
容量
在庫状況
単価
数量
Supelco
フロリジル®, PR grade, 60-100 mesh, coarse powder
カタログ番号
容量
在庫状況
単価
数量
Supelco
フロリジル®, 100-200 mesh, fine powder
カタログ番号
容量
在庫状況
単価
数量
Supelco
クロマトグラフィー用Florisil®吸着剤, 60-100 mesh
カタログ番号
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在庫状況
単価
数量
Supelco
フロリジル®, <200 mesh, fine powder
カタログ番号
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在庫状況
単価
数量
Supelco
フロリジル®, for the determination of hydrocarbon acc. to ISO 9377-2
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在庫状況
単価
数量
Supelco
フロリジル®, 30-60 mesh
カタログ番号
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在庫状況
単価
数量
Supelco
フロリジル® PR, PR grade (analysis acc. to FDA)
カタログ番号
容量
在庫状況
単価
数量