コンテンツへスキップ
Merck
  • Regulation of eumelanin/pheomelanin synthesis and visible pigmentation in melanocytes by ligands of the melanocortin 1 receptor.

Regulation of eumelanin/pheomelanin synthesis and visible pigmentation in melanocytes by ligands of the melanocortin 1 receptor.

Pigment cell & melanoma research (2008-07-17)
Elodie Le Pape, Kazumasa Wakamatsu, Shosuke Ito, Rainer Wolber, Vincent J Hearing
要旨

The production of melanin in the hair and skin is tightly regulated by the melanocortin 1 receptor (MC1R) whose activation is controlled by two secreted ligands, alpha-melanocyte stimulating hormone (alphaMSH) and agouti signal protein (ASP). As melanin is extremely stable, lasting years in biological tissues, the mechanism underlying the relatively rapid decrease in visible pigmentation elicited by ASP is of obvious interest. In this study, the effects of ASP and alphaMSH on the regulation of melanin synthesis and on visible pigmentation were assessed in normal murine melanocytes and were compared with the quick depigmenting effect of the tyrosinase inhibitor, phenylthiourea (PTU). alphaMSH increased pheomelanin levels prior to increasing eumelanin content over 4 days of treatment. Conversely, ASP switched off the pigment synthesis pathway, reducing eu- and pheo-melanin synthesis within 1 day of treatment that was proportional to the decrease in tyrosinase protein level and activity. These results demonstrate that the visible depigmentation of melanocytes induced by ASP does not require the degradation of existing melanin but rather is due to the dilution of existing melanin by melanocyte turnover, which emphasizes the importance of pigment distribution to visible color.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
エチルアルコール(純粋), for molecular biology
Sigma-Aldrich
2-メルカプトエタノール, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Sigma-Aldrich
2-メルカプトエタノール, ≥99.0%
Sigma-Aldrich
エタノール, JIS special grade, ≥99.5%
Sigma-Aldrich
2-メルカプトエタノール, BioUltra, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
Reagent Alcohol, reagent grade
Sigma-Aldrich
エタノール, SAJ first grade, ≥99.5%
Sigma-Aldrich
エチルアルコール(純粋), 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
エタノール, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
Sigma-Aldrich
エタノール, puriss. p.a., absolute, ≥99.8% (GC)
Sigma-Aldrich
エタノール, ≥99.5%, suitable for HPLC
Sigma-Aldrich
エタノール, JIS special grade, 94.8-95.8%
Sigma-Aldrich
エタノール, ≥99.5%
Sigma-Aldrich
エチルアルコール ( 変性), reagent grade
Sigma-Aldrich
エタノール, ≥99.5%, suitable for absorption spectrum analysis
Sigma-Aldrich
エタノール, JIS first grade, 94.8-95.8%
Sigma-Aldrich
2-メルカプトエタノール, ≥99.0%
Sigma-Aldrich
Reagent Alcohol, anhydrous, ≤0.005% water
Sigma-Aldrich
エタノール, ≥99.5%, SAJ super special grade
Sigma-Aldrich
2-メルカプトエタノール, SAJ special grade, ≥99.0%
Sigma-Aldrich
エタノール, JIS 1000, ≥99.5%, for residue analysis
Sigma-Aldrich
エタノール, ≥99.5%, suitable for fluorescence
Sigma-Aldrich
エタノール, JIS 300, ≥99.5%, for residue analysis