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  • Virulence strategies of an insect herbivore and oomycete plant pathogen converge on host E3 SUMO ligase SIZ1.

Virulence strategies of an insect herbivore and oomycete plant pathogen converge on host E3 SUMO ligase SIZ1.

The New phytologist (2022-05-03)
Shan Liu, Camille J G Lenoir, Tiago M M M Amaro, Patricia A Rodriguez, Edgar Huitema, Jorunn I B Bos
要旨

Pathogens and pests secrete proteins (effectors) to interfere with plant immunity through modification of host target functions and disruption of immune signalling networks. The extent of convergence between pathogen and herbivorous insect virulence strategies is largely unexplored. We found that effectors from the oomycete pathogen, Phytophthora capsici, and the major aphid pest, Myzus persicae target the host immune regulator SIZ1, an E3 SUMO ligase. We used transient expression assays in Nicotiana benthamiana as well as Arabidopsis mutants to further characterize biological role of effector-SIZ1 interactions in planta. We show that the oomycete and aphid effector, which both contribute to virulence, feature different activities towards SIZ1. While M. persicae effector Mp64 increases SIZ1 protein levels in transient assays, P. capsici effector CRN83_152 enhances SIZ1-E3 SUMO ligase activity in vivo. SIZ1 contributes to host susceptibility to aphids and an oomycete pathogen. Knockout of SIZ1 in Arabidopsis decreased susceptibility to aphids, independent of SNC1, PAD4 and EDS1. Similarly SIZ1 knockdown in N. benthamiana led to reduced P. capsici infection. Our results suggest convergence of distinct pathogen and pest virulence strategies on an E3 SUMO ligase to enhance host susceptibility.

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Sigma-Aldrich
SIGMAFAST プロテアーゼインヒビター タブレット, For General Use
Sigma-Aldrich
抗マウスIgG (全分子)-ペルオキシダーゼ ウサギ宿主抗体, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
モノクローナル抗緑色蛍光タンパク質(GFP)抗体 マウス宿主抗体, clone GSN149, purified from hybridoma cell culture