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  • IKKe in osteoclast inhibits the progression of methylprednisolone-induced osteonecrosis.

IKKe in osteoclast inhibits the progression of methylprednisolone-induced osteonecrosis.

International journal of biological sciences (2021-04-20)
Yingjie Liu, Haojie Shan, Yang Zong, Yiwei Lin, Wenyang Xia, Nan Wang, Lihui Zhou, Youshui Gao, Xin Ma, Chaolai Jiang, Xiaowei Yu
要旨

Previous studies have described that NF-κB signaling mediated by NFκB-inducing kinase (NIK) plays a critical role of the differentiation of osteoclasts. We aim to explore the role of IKKe in methylprednisolone -induced osteonecrosis of the femoral head (ONFH). Methylprednisolone-induced ONFH mice model was successfully established, and subjected to micro computed tomography to detect the femoral head image of the mice. Bone marrow cells from experimental mice were collected and cultured. qPCR and immunoblot were performed to examine the possible signal pathways of IKKe involvement, and osteoclast-related gene expressions in IKKe+/+ and IKKe-/- cells in vitro and in vivo were examined. It was found that the levels of IKKe decreased in ONFH patients, and IKKe interacted with NIK in the NF-κB signal pathway to suppress osteoclasts via inhibiting the transcription of NIK. Furthermore, IKKe knockout promoted the osteoclastogenesis in mice model. Finally, IKKe knockout suppressed methylprednisolone-induced ONFH and pro-inflammatory responses in mice model. Our findings show a mechanism of IKKe inhibition of the progression of methylprednisolone-induced ONFH via the NIK/NF-κB pathway.

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製品内容

Sigma-Aldrich
リポポリサッカリド 大腸菌055:B5由来, purified by phenol extraction
Sigma-Aldrich
抗アクチン ウサギ宿主抗体, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
抗リン酸化IKK-ε(Ser172)抗体, from rabbit, purified by affinity chromatography