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Transcriptomic Analysis in the Sea Anemone Nematostella vectensis.

Methods in molecular biology (Clifton, N.J.) (2020-10-20)
Jacob F Warner, Eric Röttinger
要旨

The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global transcriptomic level. Transcriptomics analysis is now routinely used to detect differential expression at the genome level. Here we present the latest procedures for isolating high-quality RNA required for next generation sequencing, as well as methods and resources for quantifying transcriptomic data.

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Sigma-Aldrich
クロロホルム, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Sigma-Aldrich
10 mMトリス、pH 8.0、1 mM EDTAで飽和した25:24:1のフェノール:クロロホルム:イソアミルアルコール, Supplied with Equilibration buffer, for molecular biology