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  • Polycomb Repressive Complex 1 Generates Discrete Compacted Domains that Change during Differentiation.

Polycomb Repressive Complex 1 Generates Discrete Compacted Domains that Change during Differentiation.

Molecular cell (2017-02-06)
Sharmistha Kundu, Fei Ji, Hongjae Sunwoo, Gaurav Jain, Jeannie T Lee, Ruslan I Sadreyev, Job Dekker, Robert E Kingston
要旨

Master regulatory genes require stable silencing by the polycomb group (PcG) to prevent misexpression during differentiation and development. Some PcG proteins covalently modify histones, which contributes to heritable repression. The role for other effects on chromatin structure is less understood. We characterized the organization of PcG target genes in ESCs and neural progenitors using 5C and super-resolution microscopy. The genomic loci of repressed PcG targets formed discrete, small (20-140 Kb) domains of tight interaction that corresponded to locations bound by canonical polycomb repressive complex 1 (PRC1). These domains changed during differentiation as PRC1 binding changed. Their formation depended upon the Polyhomeotic component of canonical PRC1 and occurred independently of PRC1-catalyzed ubiquitylation. PRC1 domains differ from topologically associating domains in size and boundary characteristics. These domains have the potential to play a key role in transmitting epigenetic silencing of PcG targets by linking PRC1 to formation of a repressive higher-order structure.

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Sigma-Aldrich
抗トリメチル-ヒストンH3(Lys27)抗体, Upstate®, from rabbit
Sigma-Aldrich
抗JHDM1B抗体, from rabbit, purified by affinity chromatography
Sigma-Aldrich
抗EZH2抗体, Upstate®, from rabbit