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  • Mimetic ligand-based affinity purification of immune complexes and immunoconjugates.

Mimetic ligand-based affinity purification of immune complexes and immunoconjugates.

Journal of chromatography. B, Biomedical sciences and applications (1998-04-29)
D Xu, B Leveugle, F T Kreutz, M R Suresh
要旨

We developed a simple purification method to purify alkaline phosphatase/anti-alkaline phosphatase IgG as immune complexes using mimetic affinity chromatography wherein the antibody was either a monospecific antibody, a bispecific antibody or a commercial polyclonal IgG conjugated with alkaline phosphatase (AP-IgG) covalently. The immune complexes or conjugates were efficiently bound on the mimetic Blue A6XL column and eluted under mild conditions (5-20 mM phosphate buffer). A similar strategy of purifying peroxidase/anti-peroxidase antibody complexes was also successfully demonstrated using the mimetic Red 3 column. Mimetic affinity chromatography thus appears to be a simple method to purify the desired monospecific or bispecific antibodies from the respective hybridomas and quadromas.

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製品番号
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製品内容

Sigma-Aldrich
Mimetic Blue AP Ligand Affinity Adsorbent, suspension