コンテンツへスキップ
Merck
  • Nuclear targeting of gold nanoparticles in cancer cells induces DNA damage, causing cytokinesis arrest and apoptosis.

Nuclear targeting of gold nanoparticles in cancer cells induces DNA damage, causing cytokinesis arrest and apoptosis.

Journal of the American Chemical Society (2010-01-21)
Bin Kang, Megan A Mackey, Mostafa A El-Sayed
要旨

By properly conjugating gold nanoparticles with specific peptides, we were successful in selectively transporting them to the nuclei of cancer cells. Confocal microscopy images of DNA double-strand breaks showed that localization of gold nanoparticles at the nucleus of a cancer cell damages the DNA. Gold nanoparticle dark-field imaging of live cells in real time revealed that the nuclear targeting of gold nanoparticles specifically induces cytokinesis arrest in cancer cells, where binucleate cell formation occurs after mitosis takes place. Flow cytometry results indicated that the failure to complete cell division led to programmed cell death (apoptosis) in cancer cells. These results show that gold nanoparticles localized at the nuclei of cancer cells have important implications in understanding the interaction between nanomaterials and living systems.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
金ナノ粒子, 15 nm diameter, OD 1, stabilized suspension in citrate buffer
Sigma-Aldrich
金ナノ粒子, 15 nm diameter, OD 1, stabilized suspension in 0.1 mM PBS, reactant free
Sigma-Aldrich
金ナノ粒子, 200 nm diameter, stabilized suspension in 0.1 mM PBS, reactant free