G005619

LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis^® Express C18 after Supel^™ QuE PSA/C18 QuEChERS Cleanup

application for HPLC, application for SPE

特性

• テクニック: HPLC: suitable; solid phase extraction (SPE): suitable
• 試験パラメータ: sample preparation: Dispersive (QuEChERS); sample/matrix: orange juice spiked with carbendazim at 1 ppb; extraction tube: Supel^™ QuE Citrate (EN) Tube (55227-U); extraction process: add 10 mL of orange juice with pulp to a 50 mL empty extraction tube (55248-U); add carbendazim at 1 ppb; add 10 mL acetonitrile; (shake for 1 minute; add contents of citrate extraction tube (55227-U); shake for 1 min; centrifuge at 3200 rpm for 5 minutes); clean-up tube: Supel-Q PSA/C18 (55288-U); clean-up process: transfer 0.7 mL of the acetonitrile layer to the PSA/C18 cleanup tube (55288-U); shake 1 minute; (centrifuge for 3 minutes at 3400 rpm; remove 0.25 mL of the supernatant; mix with 0.25 mL water for LC-MS analysis); column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U); mobile phase: [A] 10 mM ammonium acetate in water; [B] 10 mM ammonium acetate in methanol; gradient: 0-1 min: 30% B; 1.5-3.5 min: 100% B; 3.5-7 min: 30% B; flow rate: 0-1 min: 0.3 mL/min; 1.5-7 min: 0.5 mL/min; column temp.: 30 °C; detector: MS, ESI(+), MRM, m/z 192/160, 192/132; injection: 5 μL
• 適合性: application for HPLC; application for SPE
• アプリケーション: food and beverages

詳細

アナリシスノート

Simple and fast extraction and analytical method were developed for analysis of fungicide carbendazim in orange juice samples. QuEChERS extraction method was used with PSA/C18 cleanup. Ascentis Express C18 column chromatography was coupled to MS/MS detection with limits of detection at 0,1 ppb.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Supel is a trademark of Sigma-Aldrich Co. LLC

アナライト

Carbendazim, PESTANAL^®, analytical standard