SRP6258

グランザイムB ヒト

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• 別名: SECT, グランザイム-2, グランザイムB
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in HEK 293 cells
• タグ: 6-His tagged (C-terminus)
• アッセイ: ≥95% (SDS-PAGE)
• 形状: lyophilized powder
• 分子量: calculated mol wt 26.4 kDa; observed mol wt 35 kDa (DTT-reduced. Protein migrates due to glycosylation. Ile 21 is the predicted N-terminus.)
• 包装: pkg of 10 μg
• 不純物: <1 EU/μg endotoxin (LAL test)
• UniProtアクセッション番号: P10144
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... GZMB(3002)

詳細

詳細

Granzyme B (GZMB) also known as Cathepsin G-like 1 (CTSGL1), Cytotoxic T-lymphocyte proteinase 2 (Lymphocyte protease), Fragmentin-2, Granzyme-2, Human lymphocyte protein (HLP), SECT, CGL1, CSPB, CTLA1, GRB, which belongs to the peptidase S1 family. Granzyme subfamily GZMB / CTSGL1 contains 1 peptidase S1 domain. The catalytic activity of Granzyme B is “Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-“, and is inactivated by the serine protease inhibitor diisopropyl fluorophosphates. GZMB is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. GZMB seems to be linked to activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution and cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

物理的形状

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

再構成

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable