SRP0689

TAU-441, GSK3BETA-Phosphorylated

recombinant

• 別名: Phosphorylated Tau Protein
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in E. coli
• アッセイ: 70-95% (SDS-PAGE)
• 形状: frozen liquid
• 分子量: ~64-68 kDa
• 濃度: 0.2 μg/μL
• アクセッション番号: P10636-8
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... MAPT(4137)

詳細

生物化学的／生理学的作用

Recombinant human tag-free Tau-441 was co-expressed with GSK3beta in E. coli cells. The Tau-441 protein was phosphorylated by GSK3beta in vivo and in vitro prior to the final chromatography purification. Tau-441 or Tau-F is a member of the Tau family of proteins which function to stabilize the microtubules by binding to them. Tau proteins are subject to phosphorylation and this phenomenon regulates the association of the Tau protein with the microtubules ⁽¹⁾. Deposits of Alzheimer′s disease AD-associated proteins, such as hyperphosphorylated Tau, as well as other shared misfolded proteins, such as, beta-amyloid precursor protein (betaAPP), ubiquitin, and various chaperones and protein kinases are thought to play a pathologic role in the cognitive decline and muscular failure. Malfunctioning of Tau proteins is associated with microtubules disintegration and collapsing of the neuronal transport system ⁽²⁾.

再構成

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

保管および安定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable