SAB4200360

Anti-Cyclophilin B antibody, Mouse monoclonal

clone CYPB-3, purified from hybridoma cell culture

• 別名: Monoclonal Anti-CYP-S1, Monoclonal Anti-CYPB, Monoclonal Anti-OI9, Monoclonal Anti-PPIB, Monoclonal Anti-SCYLP, Monoclonal Anti-peptidylprolyl isomerase B
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: CYPB-3, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~20 kDa
• 化学種の反応性: human, mouse, rat
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• 濃度: ~1.0 mg/mL
• テクニック: western blot: 2.5-5.0 μg/mL using whole cell extracts of human HepG2 or rat NRK cells.
• アイソタイプ: IgG1
• UniProtアクセッション番号: P23284
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPIB(5479) ; mouse ... Ppib(19035) ; rat ... Ppib(64367)

詳細

詳細

Cyclophilin B belongs to the cyclophilin family of immunophilins. It is present in the cytoplasm, nucleus, endoplasmic reticulum (ER)/golgi and plasma membrane. The protein can also be secreted from cells. The associated gene is mapped to human chromosome 15q22.

Monoclonal Anti-Cyclophilin B (mouse IgG1 isotype) is derived from the hybridoma CYPB-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human Cyclophilin B, conjugated to keyhole limpet hemocyanin (KLH). Cyclophilin B (CypB) is a 21 kDa protein encoded by the peptidylprolyl isomerase B (PPIB) gene that belongs to the cyclophilin family, is a highly conserved class of proteins originally identified as cellular binding proteins for the immunosuppressive drug cyclosporin A. CypB is mainly located within the endoplasmic reticulum (ER) of all cell types.

免疫原

synthetic peptide corresponding to the C-terminal region of human Cyclophilin B, conjugated to KLH. The corresponding sequence is identical in mouse, monkey and canine and differs by one amino acid in rat Cyclophilin B.

アプリケーション

Monoclonal Anti-Cyclophilin B antibody produced in mouse has been used in immunoblotting and immunofluorescence.

生物化学的／生理学的作用

Cyclophilin B (CypB) is involved in inflammation, viral infection and cancer. It also plays an important role in protecting cells against endoplasmic reticulum (ER) stress via its PPIase activity. Mutations in the PPIB gene give rise to recessive forms of osteogenesis imperfecta and knockdown of this gene decreases cell growth, proliferation and migration.

In endoplasmic reticulum (ER), Cyclophilin B works as a chaperone for exported proteins. It also participates in the intracellular transport of collagen and proper formation of collagen fibril. Cyclophilin B can bind to the Gag protein of human immunodeficiency type 1 (HIV-1) and helps in HIV replication. It is also involved in the infection of hepatitis C virus (HCV), Japanese encephalitis virus (JEV) and human papillomavirus type 16 (HPV16). Cyclophilin B protects against ER-mediated hypoxia-induced cell death. It is overexpressed in various malignancies.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable