GE28-9042-70

illustra^™ Plasmid Mini (250)

Cytiva 28-9042-70, pack of 1 × 250 pieces

• UNSPSCコード: 41105501
• NACRES: NA.52

特性

• 包装: pack of 1 × 250 pieces
• メーカー／製品名: Cytiva 28-9042-70

詳細

詳細

PlasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification.

The extracted plasmid DNA resulting from performing a miniprep is called a "plasmid miniprep" and is often performed using a plasmid purification kit. Minipreps are used in the process of molecular cloning to analyze bacterial clones. When bacteria are lysed under alkaline conditions, both chromosomal DNA and protein are denatured, but the plasmid DNA remains stable. On addition of acetate-containing neutralization buffer the larger and less supercoiled genomic DNA and proteins precipitate, but the small bacterial DNA plasmids stay in the solution.

The typical plasmid DNA yield from a fresh 1.5 mL culture of E. coli containing a high copy number plasmid is approximately 9 μg. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane.

The kit contains spin columns prepacked with a novel silica membrane, suspension, lysis, and neutralization buffers containing chaotropic salts, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

特徴および利点

• Produces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.
• Total preparation time of less than 10 min represents a 50% reduction in protocol time
• Increased ease of use provided by minimal changes in pipetting volume from one part of the protocol to the next
• Fewer centrifugation stepsColor-coded tubes and columns simplify the protocol
• No organic solvents such as phenol chloroform

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

illustra is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H302,H319,H315
• 注意書き: P280 - P270 - P301 + P312 - P305 + P351 + P338 - P501
• 保管分類コード: 8B - Non-combustible corrosive hazardous materials