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Merck

GE28-4082-55

HiTrap® MabSelect

Cytiva 28-4082-55, pack of 1 × 5 mL

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

包装

pack of 1 × 5 mL

メーカー/製品名

Cytiva 28-4082-55

保管条件

(20% Ehtanol)
(20% Ethanol)

パラメーター

<20 mL/min flow rate
42 psi

ベッドサイズ

16 mm × 25 mm

ベッド容積

5 mL

カラム内径

16 mm

マトリックス

highly cross-linked agarose

平均部品サイズ

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12

working range

3-10

キャパシティ

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

適合性

suitable for bioprocess medium

保管温度

2-8°C

詳細

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

アプリケーション

Lab Purification using the MabSelect Protein A tag for capture of antibodies.

特徴および利点

  • HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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資料

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

プロトコル

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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